In order to determine the specific absorption coefficients of Z‐isomers, the specific absorption coefficient of all E‐isomer was preliminarily measured in the mobile phase consisting of CH 3 CN, MeOH, and MTBE (33.75:11.25:55, V/V/V), which was the C30‐HPLC mobile phase composition when all E‐isomer passed through the flow cell of PDA on analytical C30‐HPLC.

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This PowerPoint presentation on “Introduction to High-Performance Liquid Chromatography” is a brief and concise introduction to the components of HPLC. It is important to understand the individual component parts and how each one contributes to the overall reliability of the analysis. Microsoft PowerPoint - C4_HPLC.ppt [Compatibility Mode] Author: Jose Created Date: 10/25/2011 4:18:41 PM High Performance Liquid Chromatography (HPLC) An analytical separation technique that involves the high-pressure flow of a liquid through a column that contains the stationary phase. Stationary phase: Can be a solid (LSC) or a liquid (LLC) A mixture of compounds injected at one end of the column separates as the compounds pass through. •HPLC has been around for about 35 years and is the largest separations technique used •The history of HPLC: •Beginning of the 60’s: start of HPLC as High Pressure Liquid Chromatography •End of the 70’s improvements of column material and instrumentation –High Performance Liquid Chromatography PPT HPLC training. mardexis Posts: 148 Joined: Thu Nov 02, 2006 10:39 pm Location: Pleasanton, ca.

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(2001) Major Separation Modes of HPLC A Review There are four major separation modes that are used to separate most compounds: ¾Reversed-phase chromatography (most popular) ¾Normal-phase and adsorption chromatography ¾Ion exchange chromatography ¾Size exclusion chromatography ….Let’s look briefly at each mode Solvent and reagent - HPLC grade Mixed mobile phase or buffer Volume contraction effects – mixing water-miscible solvents pH adjustment, addition of non -ionic additives Filter immediately before use Pore size 0.45um to 0.8um Types of filter: Nylon – hydrophilic (high content of water/aqueous) PTFE – hydrophobic, chemically resistant HPLC seminar 1. Introduction High Performance Liquid Chromatography (HPLC) is one mode of chromatography, the most widely used analytical technique. – A free PowerPoint PPT presentation (displayed as a Flash slide show) on PowerShow.com - id: 474b85-ODEyZ Title: Microsoft PowerPoint - HPLC Column Troubleshooting_012909 [Compatibility Mode] Author: Owner Created Date: 1/28/2009 10:58:41 PM HPLC - HPLC The best application fields of various chromatographic modes The role of interaction types in various chromatographic modes Advantages of various chromatographic | PowerPoint PPT presentation | free to view CrystalGraphics brings you the world's biggest & best collection of hplc PowerPoint templates. WINNER!

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Here, we describe the principle of HPLC and introduce to the most important components in an HPLC system and the factors that determine the success of a measurement. Go to HPLC systems. HPLC-GPC (1) - INTRODUCTION | PowerPoint PPT presentation | free to view.

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